This poster presents an overview of recent experimental progress by the Duke DNA NanoTech Group in our efforts to create novel DNA nanostructures for computational self-assemblies as well as for fabrication of functional nano-patterned materials. We have prototyped a DNA tile type known as the 4x4 (a cross-like structure composed of four four-arm junctions) upon which we have deposited metal to form highly conductive nanowires and also are adapting multi-tile 4x4 sets for a variety of computational applications. We have recently described a DNA barcode lattice composed of DX tiles assembled on a long scaffold strand; the system propagates 1-dimensional (1D) information encoded on the scaffold strand into a specific and reprogrammable barcode pattern which is visible in 2D by atomic force microscopy. We have succeeded in demonstrating the first highly parallel computation via DNA tile self-assembly by using a single-layer superstructure made of DX tiles which computes the entire lookup table of pairwise XOR calculations up to a modest size input string length. We have prototyped a 2-state DNA lattice assembly containing actuator components and demonstrated its ability to be controllably switched between the two states. We are currently working on a molecular robotics experiment aimed at demonstrating unidirectional motion of a small DNA fragment along a track constructed of DNA. We have demonstrated a diverse set of novel structures and applications which extend the inherent information carrying capacity of DNA in a variety of novel directions.
D. Liu, S- H. Park, J. H. Reif, and T.H. LaBean (2004) DNA nanotubes self-assembled from TX tiles as templates for conductive nanowires. Proc. Nat. Acad. Sci., USA101, 717-722.
H. Li, S- H. Park, J. H. Reif, T. H. LaBean, and Hao Yan, (2004) DNA Templated Self-Assembly of Protein and Nanoparticle Linear Arrays, J. Am. Chem. Soc.126, 418-419.
H. Yan, S.H. Park, G. Finkelstein, J.H. Reif, and T.H. LaBean (2003) DNA-Templated Self-Assembly of Protein Arrays and Highly Conductive Nanowires. Science301, 1882-1884.
Yan, H., LaBean, T.H., Feng, L., and Reif, J.H. (2003) Directed nucleation assembly of DNA tile complexes for barcode-patterned lattices, Proc. Nat. Acad. Sci., USA100, 8103-8108.
Yan, H., Feng, L., LaBean, T.H., and Reif, J.H. (2003) Parallel Molecular Computation of Pair-wise XOR Using DNA "String Tile" Self-Assembly, J. Am. Chem. Soc., 125, 14246-14247.