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Addressable Self-Assembling Branched Nucleoprotein Biostructures

Jarrod Clark, Taras Chevchuok, and Steven S. Smith*

City of Hope,
Duarte, CA 91010 USA

This is an abstract for a presentation given at the
10th Foresight Conference on Molecular Nanotechnology

 

Both branched and linear DNA scaffolds can be used in the methyltransferase-directed targeting of fusion proteins. These self-assembling biostructures are easily studied, and microfluidic-chip-based analysis greatly facilitates their detection and analysis. Our experience with these systems suggests that the technology is only limited by the yield of high occupancy structures. Linear and branched biostructures containing three targeted proteins have been reliably prepared. This permits the construction of a variety of open and closed assemblies that can be designed as static or dynamic systems. Macromolecular structure-locks, rotaxanes, carcerands, and switches may find a variety of applications in biology, nanoscience and nanotechnology.

References:

  1. Smith, S.S.  Designs for the self-assembly of open and closed macromolecular structures and a molecular switch using DNA methyltransferases to order proteins on nucleic acid scaffolds. Nanotechnology 13: 413-419, 2002
  2. Smith, S.S.  A Self-Assembling Nanoscale Camshaft: Implications for Nanoscale Materials and Devices Constructed from Proteins and Nucleic Acids. Nano Lett. 1:51-56, 2001
  3. Smith S.S. Construction of Nucleoprotein-Based Assemblies Comprising Addressable Components for nanoscale assembly and Nanoprocessors. U.S. Patent # 62000782  Issued March 13, 2001
  4. Smith, S.S., Niu, L., Baker, D.J., Wendel, J.A., Kane, S.E. and Joy, D.S.  Nucleoprotein-based Nanoscale Assembly.  Proc. Natl. Acad. Sci. U.S.A  94:2162-2167, 1997.

*Corresponding Address:
Steven S. Smith
City of Hope
1500 E. Duarte Rd., Duarte, CA 91010 USA
Phone: 626-301-8316 Fax: 626-301-8972
Email: ssmith@coh.org
Web: http://ctb.coh.org



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