Addressable Self-Assembling Branched Nucleoprotein Biostructures
City of Hope,
Duarte, CA 91010 USA
This is an abstract
for a presentation given at the
10th
Foresight Conference on Molecular Nanotechnology
Both branched and linear DNA scaffolds can be used in the
methyltransferase-directed targeting of fusion proteins. These self-assembling
biostructures are easily studied, and microfluidic-chip-based analysis
greatly facilitates their detection and analysis. Our experience with these
systems suggests that the technology is only limited by the yield of high
occupancy structures. Linear and branched biostructures containing three
targeted proteins have been reliably prepared. This permits the construction
of a variety of open and closed assemblies that can be designed as static
or dynamic systems. Macromolecular structure-locks, rotaxanes, carcerands,
and switches may find a variety of applications in biology, nanoscience
and nanotechnology.
References:
- Smith, S.S. Designs for the self-assembly of open
and closed macromolecular structures and a molecular switch using DNA methyltransferases to order proteins on nucleic acid scaffolds. Nanotechnology 13: 413-419, 2002
- Smith, S.S. A Self-Assembling Nanoscale Camshaft:
Implications for Nanoscale Materials and Devices Constructed from Proteins
and Nucleic Acids. Nano Lett. 1:51-56, 2001
- Smith S.S. Construction of Nucleoprotein-Based Assemblies
Comprising Addressable Components for nanoscale assembly and Nanoprocessors.
U.S. Patent # 62000782 Issued March 13, 2001
- Smith, S.S., Niu, L., Baker, D.J., Wendel, J.A., Kane,
S.E. and Joy, D.S. Nucleoprotein-based Nanoscale Assembly.
Proc. Natl. Acad. Sci. U.S.A 94:2162-2167, 1997.
*Corresponding Address:
Steven S. Smith
City of Hope
1500 E. Duarte Rd., Duarte, CA 91010 USA
Phone: 626-301-8316 Fax: 626-301-8972
Email: ssmith@coh.org
Web: http://ctb.coh.org
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