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Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection

Chad A. Mirkin*

Department of Chemistry, Northwestern University
Evanston, IL 60208-3113 USA

This is an abstract for a presentation given at the
10th Foresight Conference on Molecular Nanotechnology

 

Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surface-enhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of gray-scale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow- band spectroscopic fingerprint. Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar.


*Corresponding Address:
Chad A. Mirkin
Department of Chemistry, Northwestern University
2145 Sheridan Road, Evanston, IL 60208-3113 USA
Phone: (847) 467.7302 Fax: (847) 467.5123
Web: http://www.chem.nwu.edu/~mkngrp/



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