Foresight Nanotech Institute Logo

« Go Back

You are viewing
Foresight Archives

Image of nano

Manipulating DNA Molecules in Synthetic Environments by Motor Proteins and Microtubules

Stefan Diez*, 1, Cerasela Dinu1, Cordula Reuther1, Ralf Seidel2, Joerg Optiz2, Michael Mertig2, Wolfgang Pompe2, and Joe Howard1

1Max-Planck-Institute of Molecular Cell Biology and Genetics,
Dresden 01307 Germany

2Max-Bergmann-Center of Biomaterials,
Dresden, Germany

This is an abstract for a presentation given at the
11th Foresight Conference on Molecular Nanotechnology


Biological molecules like DNA can be used as components in nanometer-scale devices such as electrical nanocircuits (1-3). However, fabrication of such devices poses serious difficulties for conventional manufacturing methods. To circumvent these difficulties, we are exploring novel approaches to pattern DNA on engineered surfaces using biomolecular motors. Our experimental method is based on a gliding motility assay, where microtubules are propelled over the surface of a substrate by kinesin motor proteins. The potential to use such motile microtubules as molecular shuttles for the transport of nanocargo (such as streptavidine coated beads) has been explored recently and is reviewed in (4).

Here, we report on the feasibility to not only transport but also to mechanically manipulate nanostructures by the forces of kinesin. In particular, we demonstrate the simultaneous stretching of multiple λ-phage DNA molecules between a substrate surface and moving microtubules. For that, the DNA molecules are functionalized on one of their ends such that a strong binding to predetermined contact points (e.g. gold patterns on a glass or silicon substrate) is possible. The other end of the DNA is biotinylated, and can be readily picked up by biotinylated microtubules via a streptavidine linkage. The forces produced by such a kinesin-driven system are strong enough to stretch the DNA molecules to their full contour length of 17 µm. The motile microtubules are thus employed as forceful grippers in the nanometer range. In contrast to conventional nanomanipulation methods these grippers are small and their work can be highly parallelized. By combining this approach with previously described methods to guide the movement of microtubules along predefined tracks, we aim to construct large area networks of stretched DNA molecules as templates for subsequent metalization and use as nanoelectronic circuits.


(1) E. Braun, Y. Eichen, U. Sivan, G. Ben-Yoseph: DNA-templated assembly and electrode attachment of a conducting silver wire, Nature 391, 775-778 (1998).
(2) M. Mertig, R. Seidel, L. Colombi Ciacchi, W. Pompe: Nucleation and growth of metal clusters on a DNA template, AIP Conference Proceedings 633, 449-453 (2002).
(3) M. Mertig, L. Colombi Ciacchi, R. Seidel, W. Pompe, A. De Vita: DNA as a selective metallization template, NanoLetters 2, 841-844 (2002).
(4) H. Hess, V. Vogel: Molecular Shuttles based on motor proteins: active transport in synthetic environments, Reviews in Molecular Biotechnology, 82, 67-85 (2001).

Abstract in Microsoft Word® format 22,342 bytes

*Corresponding Address:
Stefan Diez
Max-Planck-Institute of Molecular Cell Biology and Genetics
Pfotenhauerstr 108
Dresden 01307 Germany
Phone: +49-351-210-2521 Fax: +49-351-210-2000


Foresight Programs


Home About Foresight Blog News & Events Roadmap About Nanotechnology Resources Facebook Contact Privacy Policy

Foresight materials on the Web are ©1986–2017 Foresight Institute. All rights reserved. Legal Notices.

Web site developed by Stephan Spencer and Netconcepts; maintained by James B. Lewis Enterprises.