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Protein-antibody interactions measured by SFM (Scanning Force Microscopy)

Ascoli C., Loi S., Ruffa M., Baschieri P., Cappella B., Cercignani G., Frediani C.*, Pelosi P., Petracchi D.

C.N.R.-Istituto di Biofisica, Pisa, Italy

This is an abstract for a presentation given at the
Seventh Foresight Conference on Molecular Nanotechnology.
There will be a link from here to the full article when it is available on the web.

 

Specific interactions between Odorant Binding Protein (OBP) and its polyclonal antibodies have been evaluated from force-distance curves acquired by a dedicated Scanning Force Microscope (SFM) with a functionalized tip. Several control experiments have been performed to verify the specificity of the interactions. From the analysis of the force-distance curves, a mean rupture force of 50 pN has been calculated for individual antibody-antigen complexes. Moreover both the bond formation and the specific detachment have been studied as function of the contact time between the tip and the sample and the number of antibody molecules on the tip has been evaluated.


*Corresponding Address:
Carlo Frediani
C.N.R. - Istituto di Biofisica
Via San Lorenzo 24, Pisa 56127 ITALY
Phone: +39 050 513233; Fax: +39 050 553501
E-mail: frediani@ib.pi.cnr.it



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