Whereas nanotopography and electrical properties of micro and nano patterned surfaces can be characterized by SXM techniques, there is a lack of characterization methods of chemical surface states with high lateral resolution. There is a need for methods with different strengths of interactions between probes and surfaces.
We developed a set of surface labeling methods covering the binding strength spectrum from weak physisorption, over H-bridge bonds up to strong covalent bonds. These methods supply resolution from the light microscopy level down to a few nanometers. Fluorescence labeling techniques including strong chemical attachement as well as reversible adsorption were applied to micropatterned molecular monofilms with spatial resolution down to about 1 Ám. Labeling by chemically functionalized metallic nanobeads were used to obtain chemical information from lithographically patterned surfaces with lateral dimensions in the sub micron and in the nanometer range. Specifity, reversibility and resolution will be discussed based on optical, SEM and AFM measurements.
J. Michael Koehler
Institute for Physical High Technology
Winzerlaer Str. 10
Jena, Thuringia D-07745 Germany