An automated design process folds arbitrary meshes to produce DNA origami structures difficult to design by previous methods, including more open structures that are stable in ionic conditions used in biological assays.
Archive for the 'Nanobiotechnology' Category
Nanobreadboards made of DNA bricks provide twice the positional precision, twice the packing density, and faster prototyping than do alternative means to arrange functional molecules.
Recent research demonstrates that certain non-aqueous solvents can not only be used to assemble DNA nanostructures, but offer certain advantages over conventional aqueous solvents.
To educate potential entrepreneurs on strategies for moving discoveries from the benchtop to successful commercialization, Foresight co-sponsored an event in the “Ph.D. to Startup” Workshop Series of the Berkeley Postdoc Entrepreneur Program.
Technology developed by Nanobiosym, founded by Anita Goel, to enable personalized diagnostic testing won the Grand Prize of the Nokia Sensing XCHALLENGE in 2013, and this month was awarded the top prize in the Galactic Grant Competition.
At the 2013 Conference Joseph Puglisi described how single molecule fluorescence techniques were used to study changes in the conformation and composition of the ribosome, a large biomolecular nanomachine, during the process of translation of genetic information.
Using the enzyme DNA ligase and small DNA strands as building blocks provides an efficient and less expensive path to a large variety of DNA scaffolds and other structures.
Even without special designs and coatings to promote stability, simple DNA nanomachines can survive in human serum and blood for hours or even days, much longer than expected from previous studies using bovine serum, which has more damaging nucleases than does human serum.
At the 2013 Conference George Church presented an overview of his work in developing applications of atomically precise nanotechnology intended for commercialization, from data storage to medical nanorobots to genomic sequencing to genomic engineering to mapping individual neuronal functioning in whole brains.
Programmed assembly and disassembly of rigid 3D DNA origami objects has been achieved by designing complementary surface shapes based upon weak stacking interactions to create simple nanomachines.
Linking proteins to DNA scaffolds to produce complex functional nanostructures can require chemistry that damages protein function. A new systematic approach avoids exposing proteins to damaging conditions.
At the 2013 Conference Dean Astumian contrasted macroscopic machines at static equilibrium and molecular machines at dynamic equilibrium, and presented information ratchets and microscopic reversibility as the organizing principle of molecular machines.
DNA sequences designed to either stimulate a specific immune response or to down-regulate an undesirable response deliver superior performance when organized on nanoparticles to reach their intended cellular targets.
Gold nanotubes engineered to a specified length, modified surfaces, and to have other desirable characteristics showed expected abilities to enter tumor cells in laboratory studies, and to distribute to tissues within live mice as intended.
Single-molecule spectroscopy makes possible adding one rung at a time to a foundational rung grafted to a surface to make a long nanotube scaffold of predetermined sequence.
Positioning two or more molecules along a long DNA strand can cause the DNA molecule to adopt different shapes if the molecules interact. Quickly and cheaply separating these shapes by a simple gel electrophoresis assay provides a wealth of information about how the molecules interact.
Design and computational simulation of amyloid proteins of diverse functions from diverse sources enable the self-assembly of proteins that could provide scaffolds for diverse applications.
RNA origami brings new dimensions to nucleic acid nanotechnology by exploiting the much greater variety of RNA structural motifs (compared to DNA) to do what cannot easily be done with DNA origami, like fold into predetermined nanostructures rapidly while being transcribed.
In tests in a mouse model of advanced atherosclerosis, core-shell nanoparticles, composed of block copolymers and targeted to sites of inflammation and vascular injury, delivered a bioactive peptide that improved key properties of advanced plaques.
A commentary over at Gizmodo argues that ideas about molecular manufacturing that sounded like science fiction in 1986 now sound more like science fact.