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	<title>Comments on: Infectious virus synthesized from scratch</title>
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	<link>http://www.foresight.org/nanodot/?p=1202</link>
	<description>examining transformative technology</description>
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		<title>By: RobertBradbury</title>
		<link>http://www.foresight.org/nanodot/?p=1202#comment-2683</link>
		<dc:creator>RobertBradbury</dc:creator>
		<pubDate>Thu, 18 Jul 2002 01:20:14 +0000</pubDate>
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		<description>&lt;p&gt;&lt;strong&gt;Re:Replication Step?&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;I&#039;m going to ditto the critical comments.&lt;/p&gt;
&lt;p&gt;Viruses are not self-replicating. They rip-off much of the host&#039;s replication and raw material supply capability.&lt;/p&gt;
&lt;p&gt;However, we are developing relatively robust capabilties for adding chromosomes to bacteria, e.g. &quot;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11266572&amp;dopt=Abstract&quot;&gt;Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli&lt;/a&gt;&quot; and &quot;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11352566&amp;dopt=Abstract&quot;&gt;A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA&lt;/a&gt;&quot;. So we will soon be able to engineer bacteria in ways that are significantly beyond the current state-of-the-art.&lt;/p&gt;

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		<content:encoded><![CDATA[<p><strong>Re:Replication Step?</strong></p>
<p>I&#39;m going to ditto the critical comments.</p>
<p>Viruses are not self-replicating. They rip-off much of the host&#39;s replication and raw material supply capability.</p>
<p>However, we are developing relatively robust capabilties for adding chromosomes to bacteria, e.g. &quot;<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11266572&amp;dopt=Abstract">Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli</a>&quot; and &quot;<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11352566&amp;dopt=Abstract">A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA</a>&quot;. So we will soon be able to engineer bacteria in ways that are significantly beyond the current state-of-the-art.</p>
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		<title>By: Mr_Farlops</title>
		<link>http://www.foresight.org/nanodot/?p=1202#comment-2685</link>
		<dc:creator>Mr_Farlops</dc:creator>
		<pubDate>Wed, 17 Jul 2002 17:34:49 +0000</pubDate>
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		<description>&lt;p&gt;&lt;strong&gt;Re:Note to Creationists/Vitalists:&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Creationists should be careful. &lt;a href=&quot;http://angryflower.com/goinaf.gif&quot;&gt;They might get what they wish for&lt;/a&gt;.&lt;/p&gt;

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		<content:encoded><![CDATA[<p><strong>Re:Note to Creationists/Vitalists:</strong></p>
<p>Creationists should be careful. <a href="http://angryflower.com/goinaf.gif">They might get what they wish for</a>.</p>
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		<title>By: Practical Transhuman</title>
		<link>http://www.foresight.org/nanodot/?p=1202#comment-2684</link>
		<dc:creator>Practical Transhuman</dc:creator>
		<pubDate>Wed, 17 Jul 2002 05:11:54 +0000</pubDate>
		<guid isPermaLink="false">http://www.foresight.org/nanodot/?p=1202#comment-2684</guid>
		<description>&lt;p&gt;&lt;strong&gt;Note to Creationists/Vitalists:&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Of course scientists can create life! What do you think life is, -- some kind of &lt;strong&gt;miracle?&lt;/strong&gt;&lt;/p&gt;

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		<content:encoded><![CDATA[<p><strong>Note to Creationists/Vitalists:</strong></p>
<p>Of course scientists can create life! What do you think life is, &#8212; some kind of <strong>miracle?</strong></p>
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		<title>By: deoxyribozyme</title>
		<link>http://www.foresight.org/nanodot/?p=1202#comment-2682</link>
		<dc:creator>deoxyribozyme</dc:creator>
		<pubDate>Tue, 16 Jul 2002 17:07:48 +0000</pubDate>
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		<description>&lt;p&gt;&lt;strong&gt;Re:Replication Step?&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Not quite. The virus relies upon the host replication machinery to multiply. They patched together a very long strand of DNA, transcribed it, and injected mice with it. It wasn&#039;t quite as potent as wild type virus, but did cause paralysis.&lt;/p&gt;
&lt;p&gt;IMO, the research is both very cool and very scary.&lt;/p&gt;
&lt;p&gt;Ken&lt;/p&gt;

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		<content:encoded><![CDATA[<p><strong>Re:Replication Step?</strong></p>
<p>Not quite. The virus relies upon the host replication machinery to multiply. They patched together a very long strand of DNA, transcribed it, and injected mice with it. It wasn&#39;t quite as potent as wild type virus, but did cause paralysis.</p>
<p>IMO, the research is both very cool and very scary.</p>
<p>Ken</p>
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		<title>By: Saturngraphix</title>
		<link>http://www.foresight.org/nanodot/?p=1202#comment-2681</link>
		<dc:creator>Saturngraphix</dc:creator>
		<pubDate>Tue, 16 Jul 2002 14:57:48 +0000</pubDate>
		<guid isPermaLink="false">http://www.foresight.org/nanodot/?p=1202#comment-2681</guid>
		<description>&lt;p&gt;&lt;strong&gt;Replication Step?&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Correct me if I am wrong but&lt;br /&gt;
&lt;br /&gt;
By creating this, we have basically built a replicating system...is this not the first step we have been waiting...see if indeed we can build something so small and self replicates?&lt;br /&gt;
&lt;br /&gt;
granted its a virus...no control over it...but its a step in the right direction...right?&lt;/p&gt;

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		<content:encoded><![CDATA[<p><strong>Replication Step?</strong></p>
<p>Correct me if I am wrong but</p>
<p>By creating this, we have basically built a replicating system&#8230;is this not the first step we have been waiting&#8230;see if indeed we can build something so small and self replicates?</p>
<p>granted its a virus&#8230;no control over it&#8230;but its a step in the right direction&#8230;right?</p>
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