A new set of design rules enables constructing any wireframe nanostructure, which may lead to new medical applications and new nanomachines.
An automated design process folds arbitrary meshes to produce DNA origami structures difficult to design by previous methods, including more open structures that are stable in ionic conditions used in biological assays.
Nanobreadboards made of DNA bricks provide twice the positional precision, twice the packing density, and faster prototyping than do alternative means to arrange functional molecules.
Recent research demonstrates that certain non-aqueous solvents can not only be used to assemble DNA nanostructures, but offer certain advantages over conventional aqueous solvents.
To educate potential entrepreneurs on strategies for moving discoveries from the benchtop to successful commercialization, Foresight co-sponsored an event in the “Ph.D. to Startup” Workshop Series of the Berkeley Postdoc Entrepreneur Program.
Using the enzyme DNA ligase and small DNA strands as building blocks provides an efficient and less expensive path to a large variety of DNA scaffolds and other structures.
At the 2013 Conference George Church presented an overview of his work in developing applications of atomically precise nanotechnology intended for commercialization, from data storage to medical nanorobots to genomic sequencing to genomic engineering to mapping individual neuronal functioning in whole brains.
Programmed assembly and disassembly of rigid 3D DNA origami objects has been achieved by designing complementary surface shapes based upon weak stacking interactions to create simple nanomachines.
Linking proteins to DNA scaffolds to produce complex functional nanostructures can require chemistry that damages protein function. A new systematic approach avoids exposing proteins to damaging conditions.
Single-molecule spectroscopy makes possible adding one rung at a time to a foundational rung grafted to a surface to make a long nanotube scaffold of predetermined sequence.
Positioning two or more molecules along a long DNA strand can cause the DNA molecule to adopt different shapes if the molecules interact. Quickly and cheaply separating these shapes by a simple gel electrophoresis assay provides a wealth of information about how the molecules interact.
RNA origami brings new dimensions to nucleic acid nanotechnology by exploiting the much greater variety of RNA structural motifs (compared to DNA) to do what cannot easily be done with DNA origami, like fold into predetermined nanostructures rapidly while being transcribed.
An overview of three decades of progress in DNA nanotechnology emphasizes bringing programmed motion to DNA nanostructures, including efforts to incorporate design principles from macroscopic mechanical engineering.
Variable length single-stranded DNA springs determine how far a hinge of double-stranded DNA joining two stiff sections of DNA origami can bend.
Scaffolded DNA origami is combined with hinges of single- or double-stranded DNA to built simple machines parts that have been combined to program simple to complex motions.
Combinations of different types of DNA nanorobots, implementing different logic gates, work together to tag a specific type of cell in a living cockroach depending on the presence or absence of two protein signals.
A more general computational framework predicts the structures of 2D and 3D-curved DNA nanostructures impossible to predict using previously available computational methods. May lead to 3D-printing DNA nanostructures?
Design principles have been developed and tested to construct novel synthetic protein monomers that can self-assemble into large, open protein cages for potential use in vaccines and drug delivery.
Metal or other inorganic nanoparticles of 20 to 30-nm scale can be cast in arbitrary 3D shapes and configurations dictated by stiff, atomically precise molds constructed using scaffold DNA origami.
A new DARPA program seeks to bridge the gap from atoms to macroscale product manufacture in two steps, the first of which is from atoms to micrometer-scale feedstocks. DNA origami may be part of the solution.