Cell Engineering on Patterned Materials
Regulation of cell functions by
micropattern-immobilized biosignal molecules
Y. Ito*
Graduate School of Material Science, NAIST
This is an abstract
for a poster to be presented at the
Fifth
Foresight Conference on Molecular Nanotechnology.
The full paper is available here.
Regulation of biological cell function by artificial materials
is important for creation of new hybrid materials for medical
applications. We have previously showed that immobilized insulin
or growth factor proteins enhanced growth of anchorage-dependent
cells. In this study, the signal transduction from the
immobilized protein was clearly visualized and the possibility to
regulate cell functions through "artificial juxtacrine
stimulation" was examined using a micro-pattern-immobilized
biosignal protein.
Insulin or epidermal growth factor (EGF) was immobilized on
prescribed areas of poly(ethylene terephthalate) film by
photolithography. Various types of cells were cultured on the
pattern-immobilized film. The pattern-immobilized insulin or EGF
did not enhance cell adhesion but transduced a signal to the
cells through phosphorylation of tyrosine residues of cellular
signal proteins. Consequently only the cells on the immobilized
insulin or EGF grew in the medium. The enhancement of cell growth
was considered to be a consequence of signal transduction. This
microprocessing technique was useful to overcome the influence of
diffusible proteins in the culture system. The patterned
immobilization method provides a new valuable tool to investigate
the signal transduction and to regulate tissue formation on
matrices.
*Corresponding Address:
Yoshihiro Ito, Graduate School of Material Science, NAIST, 8916-5
Takayama-cho, Ikoma 630-01, JAPAN, ph: +81-743-72-5903,
fax:+81-743-72-5903, email: [email protected]
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